Into Unscientific Chapter 65

  Chapter 65 The fifth generation finally broke through!

   Four days later.

   Biomedical Laboratory.

   Right now.

  The entire laboratory was filled with a tense atmosphere with a little excitement, and there were always members of the research team wearing lab coats walking around quickly.

  Not to mention a lively girl like Tang Yiqiu, and a shy graduate student like Lian Ren Yongcun, a look of expectation flashed in his eyes from time to time.

  Actually, the reason for the violent mood swings of these tool people is very simple:

  After several days of overtime work, the interfering protein inducer that Xu Yun had previously conceived to attract male cockroaches to each other has been successfully born!

  Now the research task of the entire research group has come to the final and most critical stage:

   Synthesize the inducer and the fourth-generation imidacloprid through the combined formula deduced by Xu Yun, and try to see if it can act on MC38 cells.

  MC38 cells are one of the cells at the forefront of current biological research.

  It is derived from the C57BL/6 mouse colon adenocarcinoma cell line, grows adherently and has a fibroblast morphology.

  After implanting MC38 cells into C57BL/6 mice or immunocompromised mice, tumors and metastases will form. Therefore, related technologies are mostly used for research on the occurrence and metastasis of colorectal cancer, and have become one of the common ways to verify the efficacy of tumor drugs.

   But this kind of cell is not unique to mice, it is abundant in cockroaches, dragonflies and praying mantises.

  Belongs to a type of cell with more research information in the field of gene editing.

   And this is exactly why Xu Yun locked this anchor point:

  First of all, it is related to the sodium ion channel of the cockroach, and is an important electrical signal point for sodium ions.

  Secondly, there are many relevant experimental results of various authorities in the biological world, and there is no need to spend a lot of experiments to deduce and research.

  While Tang Yiqiu and others were busy outside, Xu Yun and Qiu Sheng were also standing in the operation room, making the final preparations for the practical session:

   "Old Qiu, are you sure you can do it?

  Let me tell you, there is no error tolerance rate in this round of experiments. If you make a mistake, you have to start over from the beginning, and you can't even shake your hands—you didn't lick it last night, did you? "

   "Don't worry, I don't have time to watch Ria Yamate, who was released last week. Ugh, what are you talking about?"

  Qiu Sheng was concentrating on adjusting the equipment at first, but after subconsciously replying half a sentence, he suddenly felt that something was wrong, and glared at him angrily:

   "Come on, come on, why don't you do it, God Xu, and I'll take a notebook next to me and write it down?"

  Xu Yun smiled when he saw this, he and Qiu Sheng were very young, so he naturally knew whether the other party was really angry.

  As the saying goes, there is a specialization in the field of technology. Qiu Sheng is recognized as the number one person among HKUST's doctoral students in the field of directing group-assisted synthesis. Xu Yun's advantage field is not in this direction.

   Xu Yun's purpose of asking Qiu to come to help lies in the current link, so it is definitely impossible to replace:

   "Just kidding, hurry up and adjust the equipment, and try to start it as soon as possible.

  By the way, send me the part of Yamanote Rie Ai later, I have a friend named reader who wants to be Kang Kang. "

  Material synthesis, this word may be familiar to many people, and the ears are almost calloused.

  But in fact, most people's understanding of this word is only on the surface, not deep.

  For example, what is it like in a biological laboratory, and what is the synthesis process like?

  It is really required to describe the steps in detail in words, and I am afraid that even one percent of people who know it may not have it.

  Usually, material synthesis is generally divided into two concepts:

  Synthesis of known substances and synthesis of unknown substances.

  The operation method of the former is very simple. It is enough to find intermediates by forward synthesis analysis and reverse synthesis analysis, which belongs to high school knowledge.

   As for the synthesis of unknown substances, it is more troublesome.

   If the synthesis of unknown substances involves biological substances, it will inevitably involve polymerase chain reaction.

  Through such a reaction, the originally extremely small amount of DNA molecules can be amplified tens to millions of times.

  After design, it can be used to extract the required DNA information from a small amount of biological samples, and can achieve single-cell or even single-molecule sensitivity.

  At the same time, specific primers can also be designed to combine different DNA fragments to synthesize new DNA molecules.

  Xu Yun and others used the solid-phase chemical synthesis method this time, which uses solids such as resins as reaction substrates, on which nucleic acid or protein molecules being synthesized are connected.

  In this way, by continuously filtering and continuously supplementing different raw materials for reaction, different monomer molecules can be connected to it in turn, and the required nucleic acid or protein can be synthesized according to the target sequence.

   Twenty minutes later.

  All preparations are complete, and the synthesis officially begins.

  Xu Yun previously deduced a total of five spatial structures formed by peptide chains, but Qiu Sheng and others used only one of them:

  C=O and N－H in the peptide plane are arranged in trans, and the peptide plane is rotated 27 degrees around Cα－N1.

  Five minutes later, Xu Yun looked at Qiu Sheng:

   "Old Qiu, the biological pathway has been verified, and the target cells have been locked, shall we proceed to the next step?"

  Qiu Sheng asked without raising his head:

   "Are nucleotide monomers protected by DMT?"

"All right."

  Qiu Sheng hummed, but did not speak. He still believed in Xu Yun very much.

   Then he turned his attention to a fixed male cockroach in the operating table, and started editing with CRISPR/Cas9 technology in earnest.

CRISPR/Cas9 technology was first proposed in 1987. In 2012, two female scientists, Jennifer Doudna and Emmanuelle Charpentier, first discovered the role of endonucleases mediated by Cas proteins in the bacterial adaptive immune system. They also obtained the 2015 Breakthrough Prize in Life Sciences.

   Since then, CRISPR/Cas9 has also become the most mainstream gene editing system today.

  Qiu Sheng locked the binding point according to Xu Yun's calculation, and the target cell was MC38 cells from cockroaches. The purpose was to use hCD47-MC38HuCELL to smash the directional fragments of feedback gender cognition.

   Once this clip is smashed, the gender recognition of the cockroach will be affected.

  Under the promotion of pheromone, the previously mentioned C good ketone behavior will be carried out.

   Right now.

  The internal microcosm of that unlucky cockroach.

   This is an incomparably vast 'planet', under the yellow sky, filled with sinful scenes:

   A ball of pathogenic mycoplasma trampled a piece of cholesterol under its feet, tore it apart with a hiss, and swallowed the severed limb with a grinning grin amidst the wailing of cholesterol.

   Not far from it, another pathogen lifted a newly synthesized Ig-like membrane protein above its head, and violently threw it down the cliff.

   A little further away, a coxsackie virus wielded a double sickle, beheading a sugar variant

  The farther ones are.

  This is a world that cannot see any dawn, no fairness, no hope, no .

future.

  After the slaughter, Mycoplasma sat at the foot of the mountain, mechanically chewing the cholesterol in his mouth.

   It has done this kind of behavior countless times, and it will continue until it dies.

  However, when it was looking for the next target, a sudden change occurred.

   I don't know when, a black spot suddenly appeared on the originally yellow sky.

  The black dot was extremely small at the beginning, but in just a thousandth of a millisecond, it broke through a huge hole.

  Like a torn piece of cloth, it expanded to the extreme distance of the field of vision.

   Before Mycoplasma could react, something resembling an anchor suddenly appeared from the hole and rushed towards a fragment floating in the air.

   Less than a moment.

  The anchor precisely hooked this fragment, and hooked it out of the world at a faster speed than when it came.

  Mycoplasma, who had never seen such a situation before, stared at the situation in front of him dumbfounded.

   Just as he was about to greet his companions to inquire about the situation, a voice that pierced through the air suddenly came from his ears.

  It raised its head subconsciously and looked towards the sky.

  At this very moment, countless scorching C1galt1c1 lures burst through the gap covering the sky.

  They came to this world with flame and justice.

   "Gah?"

   As soon as the pathogenic mycoplasma uttered a strange cry with unknown meaning, it was smashed to pieces by the lure that fell from the sky.

   Actually, it's more than that.

   Right now.

  All the evils in this world are being punished by the outside world.

  In less than a moment, this sinful world is covered in flames and is coming to an end.

   In a few minutes, the macro world.

  Following a series of changes in the microscopic realm, the experimental cockroach that was put back into the eco-box suddenly opened its wings.

   With two flaps, he ran straight towards a male cockroach

   It's strange. After sleeping for more than ten hours, why do you feel sleepy again after coding for a few hours?

  (end of this chapter)

Chapter end